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The table below shows what amounts of Hydrogen Peroxide and water are needed to make the solutions. Repeat all the tests at least three times so that an average can be obtained.
Crushed potato samples of equal weight were placed in hydrogen peroxide solutions of various temperatures.
The results showed that less gas was produced as the concentration of the inhibitor rose.
It is able to speed up the decomposition of Hydrogen Peroxide because the shape of it's active site matches the shape of the Hydrogen Peroxide molecule.
This type of reaction where a molecule is broken down into smaller pieces is called an anabolic reaction. Add 4cm3 of hydrogen peroxide solution at a concentration of 20% to the other test tube. It is very important to accurately measure the amounts of Hydrogen Peroxide, Yeast and water to ensure a fair test. Pour the hydrogen peroxide solution into the test tube containing the yeast and immediately put the gas syringe bung on the end of the test tube, at the same time start the stopwatch. Bubbles should start to rise up the tube and the gas syringe will move outwards, as soon as the gas syringe passes the 30cm3 mark stop the stopwatch and note the elapsed time down to the nearest 1/10th of a second. Repeat the experiment with hydrogen peroxide concentrations of 16%, 12%, 10%, 8%, 4% and 0%.
The catalase test tests for the presence of catalase, an enzyme that breaks down the harmful substance hydrogen peroxide into water and oxygen.
If an organism can produce catalase, it will produce bubbles of oxygen when hydrogen peroxide is added to it.Increasing the temperature will also increase the heat of the molecules.Enzymes such as Catalase are protein molecules which are found in living cells.If no bubbles form, it is a negative result; this suggests that the organism does not produce catalase.Dispose of the slide in a slide disposal bin and any toothpicks in a biohazard bin.They are used to speed up specific reactions in the cells.They are all very specific as each enzyme just performs one particular reaction.As I am comparing two variables to each other, it would be wise to calculate the correlation of the two variables.To calculate the correlation, I should use Spearman’s rank correlation coefficient.Catalase is an enzyme found in food such as potato and liver.It is used for removing Hydrogen Peroxide from the cells.